Recently, the research team of Qiang Wang, an innovation team for plant protein structure and function regulation, clarified the relationship of rheological properties of pea protein isolate-amylose/amylopectin mixtures with the product qualities of high-moisture extruded meat substitutes, simultaneously from both scientific as well as application-oriented point of view. The relevant research results were published in the journal Food Hydrocolloids (IF: 9.147) with the title “Rheological properties of pea protein isolate-amylose/amylopectin mixtures and the application in the high-moisture extruded meat substitutes”.

Recently, the plant protein-based meat substitutes have aroused great attention for their benefits in terms of environment protection, animal welfares and personal health. There has been an explosion of interest in application of pea protein in meat substitutes production, for its advantages of abundant availability, high bioavailability, low sensitization and no beany flavor. Nevertheless, the formation of meat-like fibrous structures and the tenderness mouthfeel of meat substitutes within processed pea proteins and amylose/amylopectin components remains challenging. Here, the rheological properties of pea protein isolate (PPI, 90%)-amylose /amylopectin (10%) mixtures and its effect on the high moisture extrusion processing and the final product qualities, including the appearance, texture properties and the digestibility were investigated. Results showed that the addition of amylopectin could reduce the viscosity, storage and loss modulus of PPI. It could improve the degree of grafting and promote the formation of fibrous structures in extrudates, but reduced the in vitro protein digestibility. However, amylose had no significant influence on the apparent viscosity of PPI and promoted the formation of a more compact structure after heating and cooling. The addition of amylose decreased the degree of grafting in PPI extrudates and was not conducive to the fibrous structure formation. Furthermore, both amylose and amylopectin addition could significantly reduce the die pressure and torque in high-moisture extrusion processing, and decrease the color difference, hardness, springiness, and chewiness of the extrudates. These results suggested that the amylopectin  was beneficial for preparing the pea protein-based meat substitutes with rich fibrous structure.

Our PhD student Qiongling Chen and assistant researcher Jinchuang Zhang were co-first authors, and Prof. Qiang Wang as corresponding authors. This work was supported by the National Natural Science Foundation of China (31901608), the National Key Research and Development Plan of China (2016YFD0400200), and the Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2020-IFST).

Fig. 1. Schematic illustration of high moisture extrusion process.

 

Table 1. Effect of the amylose/amylopectin ratios in PPI (90%)-amylose/amylopectin (10%) mixtures on the system response parameters.

Table 2. Textural properties of the PPI (90%)-amylose/amylopectin (10%) mixture extrudates with different amylose/amylopectin ratios.

Link to the paper: https://doi.org/10.1016/j.foodhyd.2021.106732